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KMID : 0043320030260020120
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Archives of Pharmacal Research
2003 Volume.26 No. 2 p.120 ~ p.123
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Determination of Terbutaline Enantiomers in Human Urine by Capillary Electrophoresis Using Hydropxypropyl-β-cyclodextri as a Chiral Selector
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Kim KH
Seo SH/Kim HJ/Jeun EY/Kang JS/Mar WC/Youm JR
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Abstract
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A method for the determination of terbutaline enantiomers in human urine by capillary elctro phoresis has been developed. Optimum resolution was achieved using 50 Mm phosphate b uffer, Ph 2.5, containing 15 Mm of hydroxypropyl-β-cyclodextrin as a chiral selector. Urine samples were prepared by solid-phase extraction with Sep-pak silica, followed by CE. The assay was linear between 2-250 ng/Ml (R = 0.9998 for (S)- (+)-terbutaline and R = 0.9999 tool (R)-(-)-terbutaline) and detection limit was 0.8 ng/Ml. The intra-day variation ranged between 6.3 and 14.5% in relation to the measured concentration and the inter-day variation was 8.2-20.1 %. It has been applied to the determination of (S)-(+)-terbutaline and (R)-(-)-terbutaline in urine from healthy volunteer dosed with racemic terbutaline sulfate.
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KEYWORD
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